Two-photon excitation microscopy with spatial light modulator

摘要

We attempted to observe deep regions in biological samples through two-photon excitation microscopy adopting a spatial light modulator (SLM). The SLM is used for correcting spherical aberration (SA) caused by the refractive-index mismatch between the immersion medium and sample. In the observation of fluorescent beads in transparent epoxy resin, the fluorescence intensity from a scan with SA correction was 50 times that from a scan without SA correction. After that, we observed blood vessels in a mouse brain, which became transparent with a clearing agent.