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TWO-PHOTON FLUORESCENCE MICROSCOPY AT EXTREMELY LOW EXCITATION INTENSITY
TWO-PHOTON FLUORESCENCE MICROSCOPY AT EXTREMELY LOW EXCITATION INTENSITY
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机译:两个光子荧光显微镜极低激发强度
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摘要
The present disclosure pertains to two-photon microscopy, and specifically to methods and systems for optimizing the performance of entangled two-photon absorption (ETPA) microscopy. An ETPA microscope is described with time delay tunability to optimize the coincidence of entangled photons on a sample. The optimization allows for increased two-photon absorption by the sample, resulting in increased luminescence of the sample. The ETPA microscopy systems and methods described allow for nonlinear imaging using excitation energy intensities six orders of magnitude lower than comparable two-photon absorption microscopy techniques using classical light.
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