Toxicity Assay of Phenolic Compound Isolated from the Dichloromethane Extract of Silver Fern (Pityrogramma calomelanos)

摘要

Pityrogramma calomelanos is fern in the Polipodiaceae family that grows in tropical Asia including Indonesia. This research aims to determine the molecular structure of phenolic compound isolated from the dichloromethane extract of the silver fern's aerial part and toxicity activity test. The extraction, separation, and purification were conducted by maseration, vacuum liquid chromatography (VLC), and recrystallization. The identification of the molecular structure was analyzed using spectroscopic evidance, including UV-Vis, IR, and MS. Toxicity of isolate was tested using Brine Shrimp Lethality Test. From the separation, produced isolates as pale yellow powder with melting point of 167-168°C. Based on spectroscopic data, isolate was 2',6'-dihydroxy-4'-methoxydihydrochalcone. It also showed toxicity against to Artemia salina in BSLT assay with LC_(50) of 3.957 μg/mL. Thus isolate was categorized as highly toxic and had potential as an anticancer agent.