Sequence Analysis of the Gene CYP79B3 in Cabbage (Brassica Oleracea Var. Capitata)

摘要

CYP79B3 is an important cytochrome P450 monooxygenases in glucosinolate biosynthesis. Here, the Brassica oleracea var. capitata CYP79B3 (BocCYP79B3) gene sequence was obtained from Brassica database (BRAD), and preformed for sequence analysis. The BocCYP79B3 gene mapped to Scaffold000053, and contains an open reading frame of 1,632 bp that encodes a 543-amino acid protein with a calculated molecular mass of 61.54 kD and an isoelectric point (pI) of 8.59. Subcellular localization predicted the BocCYP79B3 gene was in the chloroplast. The conserved domain of the BocCYP79B3 protein is PLN02971. The CYP79B3 protein is most closely related to B. napus. The findings of the present study provide a molecular basis for the elucidation of CYP79B3 gene function in cabbage.