Synthesis of Cyanine Dyes with Fluorescence Intensity Enhanced by Formation of Cyclic Structure and Their Application as Enzyme Sensors

摘要

Fluorescent probes are very useful as sensors for environmental changes or analyte detection. Enzyme-responsive probes provide a versatile platform for monitoring biological process in live cells and in vivo. Most of conventional enzyme-activated probes are designed by masking a functional group on fluorophores or using self-immolative spacers. In this research, to aim at the enhancement of enzyme-responsiveness, we introduced linkers between fluorophore and cleavage site.