Optimization of regeneration technology on an energy crop-castor (Ricinus communis, L.)

摘要

An efficient regeneration protocol for in vitro propagation of castor through embryonic tips was developed. According to the different properties of seed germination and embryo germination, 5 explants with high regeneration ability were picked out and their stress abilities in pre-culture period were compared. Effect on bud induction of different cytokinins (BA/TDZ/ZT) and auxin (IBA) was studied. The optimal bud induction medium was MSB5 with 0.35 mg/L BA and 0.25 mg/L IBA. The rooting and transplanting stage were also optimized and transplant survival rate was improved. The final regeneration efficiency can reach 68.3%.