Methylation, Copy Number, and LOH Analysis of Chr8q24 for Discovery of Ovarian and Breast Cancer Biomarkers

摘要

The association of genetic abnormalities and specific cancers is leading to an increasing number of biomarkers that can be used for early detection of cancers, determination of treatment protocols and predicted prognoses. Additionally, the methylation state of an increasing number of genes is also producing possible predictive biomarkers for specific cancers and stages of cancer as well. To this end, we have analyzed both breast and ovarian cancers and their normal adjacent tissue using both DNA methylation analysis and genome wide array screening for LOH and copy number variations. The combination of these two methods has yielded several groupings of markers that are cancer specific as well as showing specificity for the specific tumor type. Specifically, there are increased copies in the chr8q24 region in both ovarian and breast tumor DNA and differences in the methylation between the two tumor DNAs. We will present the results of the combination of the genome wide analysis and DNA methylation results that may be used in combination to produce potential biomarkers for these two cancer types.