Optimization of the Amplicons Detection System of Loop-Mediated Isothermal Amplification on Microfluidic Compact Disk

摘要

Salmonellosis caused by the bacteria in the genus Salmonella remains the most important foodborne disease in both developing and developed countries.Since several decades various microbial detection and quantification methods have been developed.One of recently developed gene amplification method is loop-mediated isothermal amplification(LAMP)which has been developed on tube-based platform.To facilitate the detection of pathogenic diseases in remote areas, a microfluidic platform has been developed called lab-on-a-CD.This paper presents an improved endpoint detection system for LAMP on Lab-on-a-CD platform.A set of ultraviolet(UV)emitter and color sensor have been used in this detection system to detect the emission level of LAMP amplicons.A LAMP assay has been performed with Salmonella bacteria DNA and calibrated the duty cycle of UV emitter to optimize the amplicons detection system.