Expression Profile of Matrix Metalloproteinase-9, Neutrophil Gelatinase-Associated Lipocalin and Hemojuvelin in Cat with Urinary Diseases (Abstract NU24)

摘要

Renal diseases are associated with hypoxia and subsequent inflammation. The utilization of oxygen and modulation of tissue oxygenation status mainly rely on iron. Both neutrophil gelatinase-associated lipocalin (NGAL) and the hemojuvelin (HJV)-hepcidin-ferroportin axis are important mechanisms for body iron homeostasis and regulation. NGAL, a 25-kDa glycoprotein belonging to lipocalin superfamily, acts as promising renal biomarker for humans, dogs and cats. Urinary NGAL had been reported with 3 configurations, including monomer, dimer and the complex with matrix metalloproteinase-9 (MMP-9) in human and laboratory animals. It is recently characterized in canine and feline urinary disease in our research group. MMP-9 plays an important role in regulating the degradation of extracellular matrix in process such as angiogenesis, tumor growth and metastasis. Additionally it has been reported that MMP-9 involves in renal fibrosis in chronic kidney disease (CKD). In addition, HJV, the upstream modulator ofhepcidin-ferroportin axis, has been identified as a novel renal biomarker of acute kidney injury (AKI) and CKD in human renal diseases. Interestingly, both HJV and NGAL serve as modulators of inflammation, MMP-9 is involved in the expression of NGAL, and association of NGAL prevents MMP-9 auto-degradation; hence it is possible that these proteins work cooperatively in the course of feline renal diseases. To resolve this unknown issue, we aimed to characterize the overall expression profile of three factors in cats with urinary diseases.The urine samples were collected for study. Control group contained 12 cats, AKI group contained 22 cats and pyuria group included 16 cats. According to IRIS stage, 55 CKD cats were defined with 25 cats in stage 2,19 cats in stage 3 and 11 cats in stage4 respectively. First, homemade ELISA for the detection of cat MMP-9 were successfully established. Additionally, with previous reported feline NGAL ELISA, urinary MMP-9 and NGAL concentration were determined.