Biodegradation of Used Lubricant Oil Hydrocarbons Using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in Single or Mixed Cultures

摘要

Biodegradation using bacteria is one of the alternative solutions in the management of environmental contamination by using the activity of living organisms. Bacillus subtilis and Pseudomonas aeruginosa can degrade hydrocarbons. Single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 were selected for analysis of their capability to degrade hydrocarbons from used lubricant oil. Used lubricant oil at varied concentrations of 1%, 10%, 17%, and 20% were used as hydrocarbon sources. The parameters of dissolved oxygen (DO), pH, and optical density (OD) were measured. Analysis of hydrocarbon compound degradation was carried out using gas chromatography-mass spectrometry (GC-MS). The results of DO, pH, and OD were not significantly different (P> 0.05). The GC-MS results for P. aeruginosa InaCC B290 and mixed cultures suggest they are only capable of degrading low molecular weight hydrocarbons, while B. subtilis InaCC B289 is capable of degrading low and high molecular weight hydrocarbons with a total hydrocarbon reduction of 57.72%.