Direct Selection of Synthetic Binding Proteins That Recognize Post-Translationally Modified Proteins in Living Cells

摘要

Protein phosphorylation plays an important role in the regulation of protein function and many cellular processes.Aberrant phosphorylation has been shown to be a cause of cell death as well as maligination.The inhibition of aberrant phospho-modification of proteins is based on small molecules,which target the active site of kinases that are highly conserved;therefore,specificity is hard to achieve.As such,designing therapeutic antibodies which can specifically target Phosphorylated targets holds much clinical promise.Currently,generation of phospho-specific antibodies relies primarily on hybridoma technology.