Protein Profile Euthynnus Affinis Fish Submerse Alum Solution Based on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

摘要

Euthynnus affinis and other fish product are perishable food because they have protein and water more in order to long savings and high quality so food additive one of alum solution. Alum is used for high quality contents. It is a heavy metal. The aluminum has negative effect for health in particular enzymatic system and tissue. Liver and kidney first tissue has impacted because of the detoxification of the organ. This study aims for analyzing the protein profile of Euthnnus Affinis fish submerge alum solution 0%, 10%, 20% dan 30% by SDS-PAGE method. The study object used Euthynnus Affinis fish submerge alum solution by concentration 0% (X_0), 10% (X_1), 20% (X_2), dan 30% (X_3) as long as 1 hour. The result of this study showed sample X_0 (without alum) protein band mayor cunted in 225 kDa, 139 kDa, 107 kDa, 71 kDa, 40 kDa, 36 kDa, 25 kDa, 25 kDa and minor protein 23 kDa, 23 kDa, 21 kDa, 19 Kda, 15 kDa. Sample X_1 (10% Alum) showed protein band mayor cunted in 104 kDa dan 44 kDa. and minor protein 38kDa, 35 kDa, 29 kDa, 24 kDa, 22 kDa, dan 15 kDa. Sample X_2 (20% Alum) and X_3 (30% Alum) showed same character protein band minor 29 kDa, 24 kDa dan 22 kDa. The change of the sub unit protein is because of the increasing alum solution, it then changed into band protein.