Proliferation and Glucosinolates Accumulation of Broccoli Adventitious Roots in Liquid Medium

摘要

Cotyledons from 7-day-old in vitro broccoli seedling were used as explant source in adventitious root induction on MS medium supplemented with 30 g/1 sucrose, 1.6 mg/l IBA and 7 g/1 agar. Adventitious roots from cotyledons were transferred to liquid medium containing the same components as rooting medium for two weeks, then subcultured to MS medium with diferent sugar, macrominerals and casein hydrolysate concentrations. The best adventitious root growth was observed in half-strength MS medium supplemented with 40 g/1 sucrose, 600 mg/l casein hydrolysate and 1.6 mg/l IBA (growth index of 4.00 in about 14 culture days with inoculum density of 1.0 g fresh weight/ 30 ml of culture medium). The culturing process can be stopped on the 28~(th)day for root biomass and on the 35~(th) day for glucosinolates.