Study on Optimization of Extraction Method of Total Alkaloids from Zizyphi Spinosi Semen

摘要

To obtain the best appropriate method and optimum extraction conditions of total alkaloids from Zizyphi spinosi semen (ZSS), the conditions of reflux extraction were optimized by single factor experiment and orthogonal design respectively. the experiment results were as follows: 20% acetic acid resolved in ethanol as extracting solvent (EtOH : HAc = 80 : 20 (v/v)), ratio of liquid to solid = 20 : 1 (ml/g), 4.0 h, 70°C, and these conditions were optimum conditions after validation of experiment. The total alkaloids of ZSS extracted by this method could provide a powerful guarantee for studying ingredients and activities of alkaloids in ZSS.