DEVELOPMENT OF NOVEL DNA-CUPRAC COLORIMETRIC PROBE FOR DETERMINATION OF HYDROXYL RADICAL DAMAGE AND RELATED ANTIOXIDANT ACTIVITY

摘要

An unbalance between reactive oxygen species and antioxidants in favour of oxidants may cause health issues. In the study DNA was used as probe material to measure oxidative damage caused by hydroxyl radical (~·OH) and potential protective effects of certain water soluble antioxidants. ~·OH was produced by Fenton method and a modified colorimetric CUPRAC method was developed. The absorbance difference originated from the fact DNA degradation products but not the original DNA was responsive to the CUPRAC reagent.