Preparation of Autofluorescent Polyacrylamide Nanoparticles

摘要

Nowadays, tumor imaging is widely used in biomedical field. However, the materials of imaging is rarely and strict. This study developed a method for preparing autofluorescent polyacrylamide nanoparticles (NPs). The polyacrylamide NPs were treated with glutaraldehyde, and then removed excess glutaraldehyde through ultrafiltration, glycine neutralization and ethanol precipitation, respectively. Based on this method, the polyacrylamide NPs showed strong near-infrared fluorescence (NIRF) at the emission wavelength of both 720 and 820 nm. Additionally, the nanoparticles were efficiently internalized into RAW264.7 cells and displayed strong green and red fluorescence observed with fluorescence microscope. Therefore, the NPs are expected to be applied to tumor imaging in future.