Highly efficient and sensitive detection of chloramphenicol based on chemiluminescence immunoassays with functionalized Fe_3O_4@SiO_2@Au magnetic nanoparticles

摘要

To avoid the long time of dialysis process, a novel Strategy that Bovine serum albumin (BSA) was immobilized on Au-MNPs at first and afterward the CAP molecules were immobilized on the BSA in sequence has been applied to Chloramphenicol (CAP) detection with chemiluminescent immunoassays (CLIA) based on functionalized Fe_3O_4@SiO_2@Au magnetic nanoparticles. More importantly, this designed method was successfully applied to CAP detection with the highly efficient and sensitive. With the functionalized gold magnetic nanoparticles (Au-MNPs). Samples were detected utilizing the two different chemiluminescence immunoassays, with limits of detection (LODs) of 0.07 pg mL~(-1) for Method I and 0.06 pg mL~(-1) for Method II and a 50% inhibition concentration (IC50) value of 0.086 ng mL~(-1) for Method I and 0.14 ng mL~(-1) for Method II for CAP respectively. Therefore, such chemiluminence immunoassays method could be easily adapted for small molecule detection in a variety of foods using Au-MNPs to conventional ILESA immunoassays in CAP detection.