Sagittaria latifolia (Willd.)-Syzygium aromaticum (Linn.)'s Combination Attenuates Neo-intimal Thickening, Leukocytes Adhesion and Vascular Smooth Muscle Migration in Carotid Artery Ligation Model in Sprague dawley Rat

摘要

Atherosclerosis is a vascular disease that leads to Cardio Vascular Diseases (CVD). Neo-intimal formation is an early injury occurred in atherosclerosis which is characterized by leukocyte adhesion, sub-intimal thickening and vascular smooth muscle cells migration. Sagittaria latifolia-Syzygium aromaticum's combination (Kanjeng Bulatir; KB) is commonly used in rural area of Majalengka to threat CVD, although the underlying mechanism is not well understood. This study is to elucidate the effectivity of Kanjeng Bulatir in neo-intimal formation of carotid artery especially its effect in inflammation response. We induced carotid artery ligation for model of neo-intimal formation in Sprague dawley rat. The rats were divided into four groups, A: control or Sham peration (SO) without ligation, B: carotid ligation for 28 d, C: carotid ligation and KB administration for 28 d and D: carotid ligation then KB administration in 14 d to 28 d. The result showed neo-intimal thickening reduction in group C and D while B has the thickest one. Leukocyte adhesion happen in group B, C, D then decrease in group C and D. In group B, elastic lamina was destructed; leads smooth muscle cells (SMC) migrate to sub-intimal area. This study provides evidence that KB might attenuate neo-intimal formation in vascular injury by decreasing neointima thickening, leukocyte adhesion and vascular smooth muscle cells migration.