Effect of reclamation on diversity of soil bacterial community in mining subsidence area

摘要

Soil microbial diversity is critical for the maintenance of soil health and ecological balance.Here,composition and diversity of soil bacterial community in reclaimed soils and coal-excavated subsided soils were measured using 454 pyrosequencing methods.The dataset comprised a total of 13,369sequences,which were affiliated with the Proteobacteria,Acidobacteria,Actinobacteria,Chloroflexi,Bacteroidetes,Gemmatimonadetes,Planctomycetes,Nitrospirae,Firmicutes,and Verrucomicrobia.Results showed that reclaimed soils had more community abundance and diversity of bacteria than subsided soils.Differences in abundances of dominant taxonomic groups at different taxonomic levels between reclaimed and subsided soils were visible,suggesting that long-term land reclamation has considerably changed the edaphic environment of microflora,with significant influence on soil bacteria.Correlation analysis indicated that soil organic matter and total nitrogen might be important factors shaping the underlying microbial community.Application of organic amendments and mixture mode of ieguminosae and gramineae herbage proved to be effective to improve soil fertility and restore soil microbial community diversity.It demonstrates that fertilizer treatment and re-vegetation have excellent effects on soil bacterial communities,with soil characteristics controlling or mediating biogeochemical processes and microbial community structure.This study provides a comprehensive understanding on the response of bacterial community to long-term soil reclamation of abandoned mine land and provides important implications for restoration of mine eco-environment in China.