Cloning and Construction of Overexpression Vector for FaUVR8 Gene Transformation with Strawberry

摘要

Anthocyanin is an important production of horticulture plant secondary metabolism, which is tightly concerns with fruit quality. There are lots of factors regulate plant anthocyanin biosynthesis including UV, which is one of the most significant factors, however, the effect mechanism details of UV are still remained to be study. Thus, through DNA cloning technology, total RNA was extracted from the tender leaves of strawberry 'Toyonaka', according to the conserved regions of FaUVR8 from GeneBank (KU647690). The objective cDNA bands with 1410bp were obtained by RT-PCR. Re-combined vector had been constructed using PCR production and the pMDC vector via double-digestion. The result of this recombined vector showed that it was strawberry overexpression vector pMDC-FaUVR8 via vector PCR and vector double-digestion. The construction of this vector would be a basis for further investigation of UVR8 and Agrobacterium-mediated transformations.