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Impact of Lignin Content on the Sweet Sorghum Bagasse Enzymatic Hydrolysis

机译:木质素含量对甜高粱蔗渣酶水解的影响

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Lignin has been considered as an important factor to effect the enzymatic hydrolysis of lignocellulose. In this study, impact of lignin content on enzymatic hydrolysis was investigated using sweet sorghum bagasse (SSB) delignified by NaOH or Ca(OH)2 pretreatment For NaOH pretreated samples, a negative correlation between cellulose conversion rate and the residual lignin content has been found when the lignin content is between 18.93 % and 8.72 %. Further delignification of sample can't increase efficiency of enzymatic hydrolysis anymore. For Ca(OH)2 pretreatment, there is no correlation between lignin content and cellulose conversion rate. More interesting is that the lignin content of SSB pretreated with 10 % NaOH (17.32 %) is closed to that pretreated with 10 % Ca(OH)2 (16.42 %), but the cellulose conversion rate of the latter was 1.71 times of the former. While the surface lignin content of 10 % NaOH pretreated SSB was 1.52 times higher than Ca(OH)2 pretreated SSB. These results demonstrated that the impact of lignin content on enzymatic hydrolysis of NaOH and Ca(OH)2 pretreated SSB was different. The lignin removal is mainly contributed to the enzymatic hydrolysis of SSB treated with NaOH, while the contribution of Ca(OH)2 to the enzymatic hydrolysis of SSB was complex and needed to be studied further.
机译:木质素被认为是影响木质纤维素酶水解的重要因素。在这项研究中,使用NaOH或Ca(OH)2的甜心高粱Bagasse(SSB)对NaOH预处理样品进行预处理的预处理,发现了纤维素转化率与残留木质素含量之间的负相关性研究,研究了木质素含量对酶水解对酶水解的影响。已经发现纤维素转化率与残余木质素含量之间的负相关性当木质素含量为18.93%和8.72%之间时。进一步的样品的脱氨酸不能再增加酶水解的效率。对于Ca(OH)2预处理,木质素含量与纤维素转化率之间没有相关性。更有趣的是,用10%NaOH(17.32%)预处理的SSB的木质素含量被关闭,用10%Ca(OH)2(16.42%)预处理,但后者的纤维素转化率为前者1.71倍。虽然10%NaOH预处理的SSB的表面木质素含量比CA(OH)2预处理的SSB高1.52倍。这些结果表明,木质素含量对NaOH和Ca(OH)2预处理的SSB的酶水解的影响是不同的。木质素除去主要是有助于用NaOH处理的SSB的酶水解,而Ca(OH)2的促进至SSB的酶水解是复杂的,并且需要进一步研究。

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