The Inhibitory effect of Natural materials Black Tea Theaflavins on MMPs-2 and MMPs-9 activities in Dentine

摘要

The aim of the present study is to investigate the inhibitory effect of black tea theaflavins(TF) on gelatinases (MMP-2, MMP-9) in human dentine. Protein extracts from demineralized human dentine powder were treated with TFs concentration range from 0.1-1.6 mg/mL; Samples treated with EDTA was used as a positive control while untreated sample as a negative control; Zymography was utilized to assess MMP-2 and MMP-9 activities in dentin. The results demonstrated that untreated demineralized dentine showed the strongest band of MMPs activity on zymography, while EDTA treatment completely inhibited MMPs activity; MMPs activity was inhibited by TFs in a concentration dependent manner. We conclude that theafiavins inhibited MMP-2 and MMP-9 activities, suggesting that natural product theafiavins could be a potential supplementary therapy both for prevention and treatment of dentine caries.