The anti-inflammatory effects of tetrandrine-loading poly (L-lactic acid) film in vitro

摘要

In the present study, the anti-inflammatory effects of tetrandrine-loading poly (L-lactic acid) (PLLA) films were investigated in vitro. The surface characteristics of blank PLLA film and tetrandrine-loading PLLA films were examined by electron spectroscopy for chemical analysis (ESCA). The ESCA data suggested that the tetrandrine-loading PLLA films became enriched with nitrogen atoms. The MTT assay was applied to evaluate the cytotoxicity of PLLA films with RAW 264.7 cells. Production of nitric oxide (NO) was measured by the Griess colorimetric method. The gene expression levels of inducible nitric oxide synthase (iNOS) and IL-6 were detected by quantitative real-time reverse-transcription polymerase chain reaction (real-time RT-PCR). These results suggested that the anti-inflammatory effects of tetrandrine-loading PLLA films might be the results from modulating the NO/iNOS pathway and inhibiting the mRNA expression of iNOS and IL-6 in activated macrophages.