Edible bird nest (EBN) is a powerful and nutritious food usually consumed by the Chinese Community and it is considered among the most expensive animal products which are made up by salivation of swiftlets (Aerodramus fuciphagus). The other 5% to 10% are made up of foreign matters such as feathers, faecal matter and dirt. The EBN is graded based on its aesthetics as well as its cleaning processes. The aim of this study were to determine and compare EBN of different grades (A, B, C, D) in terms of proximate composition and amino acid profile, and next to enzymatically hydrolyse and determine the degree of hydrolysis (DH) and the recovery percentage of EBN hydrolysates. The enzymatic hydrolysis were performed as an alternative cleaning process of the various grades of EBN, where the glycoproteins were hydrolysed to glycopeptides, making them soluble and leaving behind other insoluble impurities. The results in this study showed that EBN contained high crude protein content: 60.59% (EBN_A), 59.50% (EBN_B), 54.29% (EBN_C) and 56.57% (EBN_D). Lower grade EBNs (EBN_C and EBN_D) has much higher ash content, i.e. impurities, compared to higher grade EBNs (EBN_A and EBN_B). In terms of amino acid profile, EBN_D showed the highest total amino acids compared to EBN_A, EBN_B and EBN_C, with serine and aspartic acid being the main amino acids. Treating the EBN with alcalase for 1.0 - 4.0 hours produced hydrolysates with different degree of hydrolysis (DH), ranging from 10.83 %DH (EBN_A) to 13.79 %DH (EBN_C). The recovery of EBN after enzymatic hydrolysis range from 89 % (EBN_B) to 99% (EBN_A). Overall, results showed nutritional composition and amino acid profile of EBN of various grades were significantly different in its nutritional quality, while the enzymatic hydrolysis has successfully separated the impurities from the lower grades EBN.
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