Migration analysis of DEHP from Inner Liner of Beer Bottle Caps by HPLC

摘要

The quality and quantity of DEHP (Di-(2-ethylhexyI) phthalate) migration from inner liner of beer bottle caps were investigated using HPLC (high performance liquid chromatography) with a PDA detector. Methanol/water (95:5 V/V) solution and ethanol were used as extracting solvents, respectively. The HPLC method involved a CIS column at 30°C, a mixture of methanol-water (95:5, v/v) as mobile phase and detection at 275nm. The results show that the extraction compound from the inner liner of beer bottle caps is DEHP. The detection limit of the method is 3ug/ml (S/N=3). Precision relative standard deviations (RSD) for lOOug/ml and 200ug/ml DEHP standard solution were 1.5% and 0.6% (n=7). In range of 10-200ug/ml linearity regression equation was 7=1789.4 LY-1701.80(Y: peak area, X: concentration, ug/ml) with linear correlation coefficient (R2) 0.9982. Recovery rates for DEHP in methanol-water (95:5, v/v) solution ranged from 97.9 to 109.3% and in ethanol from 107.0 to 114.2%, with repeatability RSD smaller than 1.0%.