Some marine microalgae, such as Isochrysis sp., produce high-melting (～70 °C) lipids known as long-chain alkenones that detrimentally affect biodiesel fuel quality. A method has been developed for the production of an alkenone-free Isochrysis biodiesel This material was prepared on sufficient scale to allow for extensive analysis accoiding to ASTM standard1. Results revealed that while cold flow improved by removal of these high-melting components and lubricity and glycerol content were below maximum levels pi escribed m the standards, other properties dependent on iatry acid methyl ester (FAME) structure and composition such as oxidative stability iailed to meet ASTM specifications Both the cetane number and kinematic viscosity were lower than what would be calculated based on FAME content This has been explained m part by 6—8% non-FAME contaminants in the tested samples
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