Differentially Expressed Apoptosis-Related Genes Profile of T47D Breast Cancer Cells Treated With Angiotensin-(1-7)

摘要

Angiotensin-(1-7) [Ang-(1-7)] is an endogenous seven-aminoacid peptide hormone of the reninangiotensinsystem that has antiproliferative properties. Recent studies suggest that Ang-(1-7) inhibits growth oflung cancer cells through reduction in cellular proliferation and induction of apoptosis. The aim of this work is toassess the pro-apoptotic effects of Ang-(1-7) in breast cancer cells (T47D). Methods: T47D cells were treatedwith angiotensin-(1-7) (10-6M) for 48 hours. The percentage of apoptotic cells was measured by flow cytometry(Millipore guava). In addition, we used the SA Biosciences Human Apoptosis RT2 Profiler PCR Array profiles toanalyze the expression of 84 key genes involved in programmed cell death. Network analyses of differentiallyexpressed genes were performed through MetaCore database (GeneGo). Ang-(1-7) caused a significantly higherrate of apoptosis than the control group. More than 60% of the apoptosis-related-genes were differentiallyexpressed, of which approximately 50% genes were up-regulated, such as TNF, BCL2, BAX and BIK, whichhave shown to be nineteen fifteen, nine and four times more expressed, respectively, than control. Furthermore,seventeen regulatory gene networks were derived from MetaCore analysis, suggesting the molecularmechanisms underlying of apoptotic-driven T47D cells. Conclusions: In summary, it is clear that Ang-(1-7)induces apoptosis in T47D breast cancer cells and it probably does so through activation of BCL2 gene familyand TNF related genes.