Nanoparticle-Encapsulated Tamoxifen Inducing Cytotoxic Effect on Mcf-7 Breast Cancer Cell Lines

摘要

Nanoparticle in drug delivery offers major improvements to those limitations by providing longer circulation times, greater specificity, and enhances anticancer effects. Thus, the objective of this study is to synthesize a polymeric nanoparticle encapsulating TMX, and investigate the in vitro characteristics on breast cancer cell line, MCF-7. Materials and Methods. The nanoparticles were synthesized through gamma irradiation polymerization between N-isopropylacrylamide (NIPAAM), N-vinyl-2-pyrrolidone (VP), poly(ethyleneglycol) diacrylate (PEG-DA). TMX was directly loaded into the nanoparticles by random physical entrapment. Photon Cross Correlation Spectroscopy Nanophox (PCCS-Nanophox) and Transmission Electron Microscopcy (TEM) were used to observe size and morphology of the nanoparticles. To study the in vitro effects, MCF-7 cells were treated with TMX-nanoparticles and its cytotoxicity effects were investigated through MTT assay. Results and Discussion. From the results, it was confirmed that nanoparticles at 0.055M subjected to 8kGy of gamma irradiation posses a consistent size distribution of 49.89 nm SD ±1.82. This was further confirmed through pictures taken with TEM in which the nanoparticles had a clearly distinguishable core-shell structure, with a spherical or elliptical shape with smooth surface. Through the cytotoxicity assay, it showed that TMX-nanoparticles had a greater inhibitory effect compared to TMX alone at 48 hrs and 72 hrs. There was also no evidence of toxicity by void polymeric nanoparticles.