A surface-enhanced resonance Raman scattering method for the determination of trace crystal violet on colloidal Au nanoparticle substrate

摘要

Colloidal Au nanoparticles as active substrate for surface-enhanced resonance Raman scattering (SERRS) were prepared by the trisodium citrate-reduced procedure. In pH 6.6 Na_2HPO_4-NaH_2PO_4 buffer solution and in the presence of aggregation reagent NaCl, nanogolds were aggregated to form stable aggregated-nanogolds (ANG). The crystal violet (CV) adsorbed on the surface of ANG to form CV-ANG conjugates that produce strongest surface-enhanced resonance Raman scattering peak at 1616 cm~(-1). In the optimal condition, the SERRS intensity at 1616 cm~(-1) was linear to the CV concentration in the range of 2.5×10~(-8) - 1.75×10~(-7) mol/L, a surface-enhanced resonance Raman scattering assay was set up for detection of trace CV, with good selectivity.