Rapid determination of 5-hydroxymethylfurfural by ultraviolet spectrophotometry in glucose diphasic hydrolysate

摘要

The content of 5-hydroxymethlfurfural (5-HMF) in glucose diphasic hydrolysate was measured by ultraviolet spectrophotometry (UV), while 5-HMF solution and distilled water was used as the standard sample and controlling blank instead of water-dimethylsulfoxide (DMSO) diphasic solution respectively, analyzing the content of 5-HMF at wavelength 284 nm. The determination results showed us a favorable linear relationship between the absorbance and the concentration of 5-HMF, when it is fall in between 0-0.10 mmol/L. This method is suitable for rapid and accurate determine 5-HMF.