Detection of Zearalenone by Chemiluminescence Immunoassay in Corn Samples

摘要

A chemiluminescence immunoassay with polyclonal antibody for detecting zearalenone in corn samples was developed. The optimized reaction conditions include the concentration of coating antigen (0.253 μg/ml), the polyclonal antibody (1:7500) and HRP labeled goat anti-mouse antibody (1:40000), the competition time (60 min), pH of dilution buffer (7.4), the concentration of methanol (10%) and the ratio of luminol to ρ-iodophenol (2:5) were all investigated. Based on optimum conditions, the chemoluminscence ELISA detect range was f 0.05 - 54.34 ng/ml, with IC50 was 2.93 ng/ml, and the limit of detection was 0.02 ng/ml. The chemiluminescence immunoassay shows a good agreement with commercial ELISA kit for detection zearalenone in the spiked samples. The chemiluminescence immunoassay is easier in pretreating, and more sensitive when compared with other detection methods include chromatography methods and immunoassays.