Influence of Chelation Rate on the Oligochitosan Preparation by Enzymolysis of CTS-Cu (II)

摘要

To prepare a homogenous low-molecular-weight chitosan oligosaccharide with high solubility and physiological activity, the CTS-Cu(II) chelate was degraded by cellulose, xylanase, semi-cellulase, pectinase, lipase. Cellulose was used to investigate the enzymolysis of the CTS-Cu(II) chelates at various chelation rates. The oligochitosan yields were determined by ultrafiltration and spectrophotometry. The molecular weight distributions of the degradation products were compared by gel column chromatography. Results showed that the generation of the CTS-Cu(II) chelate promoted chitosan degradation. Product yield can be changed by controlling the chelation rates of the CTS-Cu(II) chelates. When the CTS-Cu(II) chelate was degraded at a chelation rate of 100%, most molecular weights of the products were about 2500 Da, and the oligochitosan yield was 89.21%. All these results provided a theoretical basis and experimental method for preparing oligochitosan with a homogenous molecular weight.