Effects of advanced glycation end products on the mRNA expression of urotensin II and G protein-coupled receptor in rat mesangial cells

摘要

Objective To investigate the impact of AGEs with different concentrations and different times on the expression of U1I and GPR14 mRNA in MC. Methods In vitro rat mesangial cells, by adding different concentrations of AGEs(final concentration 0, 25, 50, 100 and 200 mg/L), 37°C incubated for 24 h,AGE100 mg/L groups were cultured 0, 2, 8, 16 and 24 h separately, established the control groups respectively, collecting MC,cxtracted total cellular RNA for RT-PCR reaction and detected the expression of UI1 and GPR14 mRNA in MC. Results The expression of UII and GPR14 mRNA in MC were increased with the change of AGEs concentrations from low to high.Compared with AGEs 0 mg / L group, the expression of UII and GPR14 mRNA in AGEs50, 100, and 200 mg / L groups were significantly higher (P<0.05); In AGEs100mg / L groups, the expression of UII and GPR 14 mRNA in MC were increased with the time extended, compared with AGEs Oh group, the expression of UII and GPR14 mRNA in AGEs8, 16, and 24h groups were significantly higher (PO.05); There were no change in control groups (P>0.05) . Conclusion AGEs can significantly increase the expression of UII and GPR 14 mRNA in MC.