The Expression of MAP2 in the Process of PC12 Cells Differentiated into Neurons

摘要

MAP2 plays a vital role in PC12 cell differentiation, expecialy the length of the synapse and the formation of microtubules. Here, NGF (Nerve Growth Factor) and OGD (oxygen-glucose deprivation) was used to stimulate PC12 cells, and convert them into neurons to establish an ischemia in vitro model. In order to further find MAP2 expression in PC12 cells. Immunocytochemical analysis and western blot were used to determinate the protein expression of MAP2. MAP2 expression was determined by western blotting after different treatment time periods. The results showed three repeated experiments. The expression of MAP2 was different in different time. Consult the relevant literature, PC12 cells can be inducted into Nerve cells by NGF. MAP2 exists in PC12 cell differentiation time point. It is closely related with the length of the synapses and microtubules formation. MAP2 plays an important role in synaptic neuron growth.