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Real-Time Kinetics Measurements of Protein Induced Conformational Changes in DNA

机译：DNA蛋白质诱导构象变化的实时动力学测量

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摘要

We combine electrical orientation of surface bound dsDNA probes with an optical technique for kinetic measurement of protein-induced conformational changes in DNA. The effects of probe surface density and protein variation are investigated.

机译：我们将表面结合的DSDNA探针的电向与光学技术相结合，用于DNA中蛋白质诱导的构象变化的动力学测量。研究了探针表面密度和蛋白质变异的影响。

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《LEOS Annual Meeting》|2009年||共2页
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Philipp S. Spuhler; Jelena Knezevic; Ayca Yalcin; Peter Droge; Ulrich Rant; M. Selim Unlu;
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专利

1. Platform for in situ real-time measurement of protein-induced conformational changes of DNA [J] . Philipp S. Spuhler, Jelena Knezevic, Ayca Yalcin, Proceedings of the National Academy of Sciences of the United States of America . 2010,第4期

机译：原位实时测量蛋白质诱导的DNA构象变化的平台
2. Real-time monitoring of protein-induced DNA conformational changes using single-molecule FRET [J] . Methods: A Companion to Methods in Enzymology . 2019,第期

机译：使用单分子褶皱的蛋白质诱导的DNA构象变化的实时监测
3. Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli [J] . Tworzydlo M, Polit A, Mikolajczak J, The FEBS journal . 2005,第5期

机译：DNA和cAMP与大肠杆菌中cAMP受体蛋白结合后诱导的构象变化的荧光猝灭和动力学研究
4. Real-time kinetics measurements of protein induced conformational changes in DNA [C] . Spuhler, Philipp S., Knezevic, Jelena, Yalcin, Ayca, IEEE LEOS Annual Meeting Conference Proceedings;LEOS '09 . 2009

机译：蛋白质诱导的DNA构象变化的实时动力学测量
5. High-throughput detection of DNA orientation and conformation for characterization of protein-DNA interactions. [D] . Spuhler, Philipp S. 2012

机译：高通量检测DNA方向和构象，以表征蛋白质-DNA相互作用。
6. Platform for in situ real-time measurement of protein-induced conformational changes of DNA [O] . Philipp S. Spuhler, Jelena Knežević, Ayça Yalçin, 2010

机译：原位实时测量蛋白质诱导的DNA构象变化的平台
7. Real-Time Kinetics Measurements of Protein Induced Conformational Changes in DNA [O] . Jelena Knezevic, Ayca Yalcin, Peter Dröge, 2015

机译：蛋白质诱导DNa构象变化的实时动力学测量
8. Kinetic Flow Dichroism Study of Conformational Changes in Supercoiled DNA Induced by Ethidium Bromide and Noncovalent and Covalent Binding of Benzo(a)pyrene Diol Epoxide [R] . Yoshida, H., Swenberg, C. E., Geacintov, N. E. 1987

机译：溴化乙锭诱导超螺旋DNa构象变化的动力学流动二向色性研究及苯并（a）芘二醇环氧化物的非共价和共价结合

Real-Time Kinetics Measurements of Protein Induced Conformational Changes in DNA

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