Induction of humoral response following immunization with three different plasmid DNA vaccines against African trypanosomiasis

摘要

African Trypanosomiasis (AT) is a parasitic disease caused by the flagellated protozoan of the species Trypanosoma brucei. Currently the treatment used to combat AT is not satisfactory because the drugs used for the treatment are potentially toxic and often associated to important secondary effects. In this study, we show that Balb-C mice intramuscularly immunized with a single dose of plasmid encoding three antigenic candidate genes from Trypanosoma brucei, named Invariant Surface Glycoprotein (ISG), trans-sialidase (TSA), and Phospholipase C (PLC) are able to produce antibodies IgG anti-trypanosoma. This immunization process is herein shown as able to control the mortality level when mice were submitted to challenge assay with Trypanosoma brucei brucei parasites. Our results open up the possibility of the use of new attractive targets for vaccine development against AT.