Glutathione mediated metabolism of the safener MG-191

摘要

In vitro metabolism of the safener MG-191 was studied by incubating the safener with glutathione and various recombinant glutathione transferases such as the maize phi class ZwGSTFl-1 and ZwGSTF3-3, the maize tau class ZwGSTUl-1 and the Arabidopsis zeta class ^4/GSTZl-l isozymes. All GSTs catalyzed the GSH-dependent dechlorination of the safener and the formation of a pyruvic aldehyde ethylene ketal was verified by GC-MS analyses. A mechanism leading through unstable glutathione conjugates for the formation of the aldehyde metabolite was suggested.