DNA-Based Approaches for Diagnosis of Fungicide Resistance

摘要

The increasing use of modern diagnostic tools to detect and quantify diseases and fungicide resistance will play an important part in improving the rational use of fungicides within integrated disease management systems. When testing fungicide resistance, bioassays including mycelial growth and spore germination tests in vitro, and inoculation tests on plants are usually employed. However, such methods are generally time-consuming, laborious, and allow relatively few fungal isolates to be monitored. Furthermore, biotrophs such as powdery mildews, downy mildews or slow-growing fungi like Venturia spp. are difficult to handle in general. Development of novel methods will therefore be required to quickly test fungicide resistance. In this article, the author will summarize the DNA-based techniques for diagnosis of fungicide resistance, which have already appeared in literatures. The suggested molecular mechanism of resistance to the newly developed strobilurin fungicides is also mentioned.