Novel Method for Monitoring Fungicide Resistance in Botrytis cinerea

摘要

Botrytis cinerea causes serious damage on many crops. Selection and timely application of fungicides are most important for the control of gray mold. To monitor the distribution of resistant strains in the field, the following selective medium for B. cinerea isolates was developed. Composition of this medium is MgSO_4.7H_2O, 0.5 g; NaNO_3, 2g; K_2HPO , lg; L-glutamine, 2g; fructose, 10g; and agar, 15g in 1000 ml of DW for basal medium. Streptomycin sulfate, 300mg; triphenyltin acetate, 1.5mg; copper oxide chloride, 25mg; Rose Bengal, 25mg were added as inhibitors. The airborne spores of B. cinerea were easily trapped on this medium by using a novel air sampler. Further, adding the fungicides to this selective medium could monitor more easily and rapidly the fungicide resistance of B. cinerea. This method was more convenient for the diagnosis of resistant strains as compared to a conventional mycelial growth method.