MORTALITY OF DIFFERENT STAGES OF ANASTREPHA LUDENS AND A. OBLIQUA IN VITRO AND IN VIVO, AND MANGO FRUIT QUALITY AFTER EXPOSURE TO CONTROLLED ATMOSPHERES (0 kPa O{sub}2 AND 50 kPa CO{sub}2) AT HIGH TEMPERATURES

摘要

The in vitro and in vivo (artificially and naturally infested in mango fruit) mortality of different stages (eggs, first and third instar larvae) of Anastrepha ludens and A. obliqua was determined after exposure to controlled atmospheres (0 kPa O{sub}2 + 50 kPa CO{sub}2) at different temperatures (35-55°C) and different exposure periods (160-240 min). CA at > 40°C for ≥ 160 min increased the mortality. The in vivo mortality was faster than the in vitro mortality. Eggs were more tolerant than larval stages. A. obliqua is slightly more tolerant than A. ludens. The mean estimated temperatures for 50%, 99%, and 99.9968% of in vitro mortality (LT50S, LT99S, LT99.9968S) of eggs of A. obliqua exposed to 0 kPa O{sub}2 + 50 kPa CO{sub}2 at 51, 54 and 55°C for 240 min were 49.4, 54.8 and 60.9°C, respectively. 'Manila' mango fruit can tolerate these CA treatments at < 44°C for 160-240 min, but fruit is injured at higher temperatures.