Selection and effects of rhizosphere-competent biocontrol bacteria on chickpea plant development and yield

摘要

A Procedure which consumes less screening time and space was developed for screening chickpea rhizosphere-competent Bacteria for supression of the chickpea pathogenic fungi Fusarium oxysporom f. s.p. ciceri, Rhizoctonia baiaticola and Pythium sp. Using this method out of the 478 bacteria one rhtrizosphere-competent biocontrol bacterium Pseudomonas fluorescent NBR11303 was selected for further work because of its unique, ability to inhibit all three fungi and good chickpea rhizosphere colonization ability. Significant growth increases in shoot length, dry weight and grain yield, averaging 11.59%, 17.58% and 22.61% respectively above untreated controls were attained in field trials in Agrand Jhansi. A raampidn-reistant mutant P. fluorescens NBR11303Rof the P. fluorescens NBR11303, used to monitor chickpea root colonization confirmed the rapid and aggressive colonization by the bacterium, making it a potential biocontrol agent against chickpea phytopathogenic fungi. The screening method should proveuseful in identifying rhizosphere bacteria with the greatest potential for controlling diseasecaused by phytopathogenic fungi.