Spirulina platensis is one of the popular species that containing C-Phycocyanin.C-Phycocyanin has been used in many applications such as antioxidant,anti-inflammatory,and natural blue colorant.The increase of phycocyanin production from S.platensis is influenced by extraction method and solvent ratio.The phycocyanin pigment can be massive extracted using mechanical cell rupture like freezing thawing method and distillated water as solvent.This research aimed to produce high concentration of antioxidant phycocyanin from S.platensis using simple and efficient method for large scale industry and develop a method for primary extraction from dry biomass of S.platensis.There were 3 treatments and each treatment was conducted in 3 replications.Standardization of process treatments such as biomass:solvent ratio (1:6,1:50 and 1:100) and processing time were carried out at thawing time (1 hour),freezing time (2 hours) and stirring time in water bath (0,5 hour).The phycocyanin concentration and yield were determined by using spectrophotometer.The result showed that at biomass:solvent ratio of 1:50,resulted C-PC concentration of 0.179 mg/ml and its yield of 17.944 mg/g.
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