Immunogenicity evaluation of recombinant fim-c salmonella typhi protein as typhoid vaccine candidate on wistar rat

摘要

Typhoid fever is a world health problem and often occurs in developing countries,including Indonesia.In a previous study,the UNJ Salmonella team successfully isolated,cloned,expressed,and purified inclusion bodies Fim-C S.typhi recombinant protein sized 31 Kilo Dalton(KDa).Furthermore,these proteins have been used as antigen in immunogenicity evaluation with ddY mice as test animals and give excellent results.This study aims to determine the immune response other of rodent test animals against Fim-C S.typhi recombinant protein as antigen.Immunogenicity evaluation was performed using male Wistar rats.That were divided into five test groups: Normal group,Control Group 1,Control Group 2,and Samples Group 1,Samples Group 2.The results of the ELISA analysis showed an increase in antibody titters produced by Wistar rats after subcutaneous immunized with Fim-C protein emulsified adjuvant or without adjuvant.The result of Western Blot showed the specific interaction between iinclusion bodies Fim-C S.typhi recombinant protein as antigen with anti-Fim-C S.typhi antibodies.It can be concluded that Fim-C S.typhi protein can be used as a potential vaccine candidate for typhoid disease.These results are expected to be an alternative in the discovery of new vaccines.