Comparison of PCR assays for reliable, early and rapid detection of PRRSV in different sample types from experimentally infected boat

摘要

Increased rates of false negative results were reported in several PRRSV RT-PCR assays commonly used in Europe and North America and the usage of multiple assays has been recommended to improve the diagnostic success for PRRSV.1,2 The aims of this study were to compare three commercial PRRSV real-time RT-PCR assays for detection of genetically diverse PRRSV isolates in serum, semen, blood swabs and oral fluids collected from experimentally-infected boars.