REPLICATION OF BIOMIMETIC NANOSRUFACES AND THEIR APPLICATION TO CELL DIFFERENTICATION STUDY

摘要

In this study, we report the influence of the biomimetic polystyrene (PS) nanosurfaces of nanopore and nanopillar arrays on osteoblast differentiation of MC3T3-E1 cells. The nanosurfaces with nanopore and nanopillar arrays were replicated by a hot embossing process with the nickel mold inserts having nanopillar and nanopore arrays, respectively. And the osteoblast differentiation of MC3T3-E1 cells on the nanosurfaces was evaluated by alkaline phosphatise (ALP) staining and RT-PCR analysis of induced ALP and osteocalcin gene expression. We observed that the nanopore arrays were most effective in promoting osteoblast differentiation of MC3T3-E1 cells.