(00583)Tissue-specific TfR Expression in Organs of Immature Mice after Chronic Exposure to CoCl_2

摘要

Cobalt (Co)has significantly increased its concentration in the environment in the last years due to anthropogenic and industrial activities. The present study was designed to elucidate the effect of chronic cobalt chloride (CoCl_2)exposure on transferrin receptor 1 (TfR1)in various organs of immature mice. Pregnant ICR mice were subjected to daily dose of 75 mg/kg body weight CoCl_2x6H_2O 2-3 days before they gave birth and treatment continued until day 25 after delivery. The compound was dissolved and administrated with drinking tap water. Age-matched mice obtaining regular tap water were used as a control group. On days 18 and 25 pups were sacrificed, blood plasma, livers, kidneys and spleens were obtained and processed for analysis. The results show altered tissue-specific TfR expression in the studied organs of CoCl_2-treated immature mice. A significant time-dependent increase in tissue Co levels was observed. Our data on the effects of chronic CoCl_2 exposure on TfR expression contribute for the elucidation of the complex regulatory mechanism of iron homeostasis.