Helical Tracks in Shaken Cylindrical Bioreactors Improve Oxygen Transfer and Increase Maximum Cell Density Obtainable for Suspension Cultures of Mammalian Cells

摘要

The introduction of a helical track into a cylindrical vessel, orbitally shaken on a horizontal platform, showed a considerable improvement in the volumetric mass transfer coefficient (k_La). In helical track vessels from 50 ml to 1000 L, compared with identically dimensioned cylindrical vessels, an almost 10 fold increase of k_La was observed for some conditions. On-line dissolved oxygen (DO) measurement in a high density cell culture of CHO DG44 cells using 1 L helical track vessels demonstrated that the DO remained above 50% with the viable cell density reaching 2.0 x 107 cells ml"1. These results suggest that helical tracks within orbital shaking reactors provide significant benefits over standard cylindrical vessels and open the possibility of using this principle for large-scale shaken bioreactors of 1000 L or more.