Computational approach for predicting sulfation pattern of heparan sulfate using high resolution tandem mass spectrometry data

摘要

Our algorithm treats different modification types sequentially and can handle the ambiguity problem and sulfo-losing problem in HS sequencing effectively (in a de novo way). Only basic assumptions required! (1) good spectrum; (2) biosynthetic rule; (3) Fair amount of assignments with high confidence. Hard to determine the specific position of sulfation on GlcN without considering about biosynthetic rules.