Online Electrochemical Reduction of the Disulfide Bond(s) in Oxytocin and Hepcidin Results in Different CID and ETD Fragmentation Spectra

摘要

In summary, the here presented study shows that disulfide bridges can be successfully reduced in an on-line set-up using electrochemistry. It will be of great interest to further study the coupling of EC with LC-based separation of proteins and on-line digestion, as this might finally lead to a fully integrated workflow overcoming the reduction/alkylation step in standard bottom-up proteomics. In addition, the coupling of EC with LC-based peptide separation may open new avenues in the field of middle-down sequencing of (large) peptides.