Proteomic analysis of salt-responsive ubiquitin-related proteins in rice roots

摘要

The expressions of ubiquitination on pyruvate phosphate dikinase 1, heat shock protein 81-1, probable aldehyde oxidase 3, plasma membrane ATPase, cellulose synthase A catalytic subunit 4 [UDP-forming] and cyclin-C1-1 was identified and analyzed before and after salt treatment. There was consistency between salt-tolerance of those three rice lines and the abundance of ubiquitinated pyruvate phosphate dikinase 1, cellulose synthase A catalytic subunit 4 and cyclin-C1-1 after salt stress. In addition, K48-linkage polyubiquitination on heat shock protein 81-1 and probable aldehyde oxidase 3 was inferred, resulting in the inferior salt-tolerance in SA0604 and TNG67. Findings in the present study demonstrate that the regulated mechanisms of protein ubiquitination were very important for rice seedlings against salt impact.